mtb erdman strain strain mtb erdman atcc 35801 (ATCC)
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mtb erdman strain strain mtb erdman atcc 35801
Mtb Erdman Strain Strain Mtb Erdman Atcc 35801, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mtb+erdman+strain+strain+mtb+erdman+atcc+35801/Mycobacterium+Tuberculosis/10__7554_slash_elife__51461-254-41-47
Average 94 stars, based on 54 article reviews
Mtb Erdman Strain Strain Mtb Erdman Atcc 35801, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mtb+erdman+strain+strain+mtb+erdman+atcc+35801/Mycobacterium+Tuberculosis/10__7554_slash_elife__51461-254-41-47
Average 94 stars, based on 54 article reviews
mtb erdman strain strain mtb erdman atcc 35801 - by Bioz Stars,
2026-09
94/100 stars
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Expressing:Article Title: Dynamic post-translational modification profiling of Mycobacterium tuberculosis-infected primary macrophages Article Snippet: Key resources table Reagent type (species) or resource Designation Source or reference Identifiers Additional information Cell line (M. musculus) RAW 264.7 ATCC TIB-71 Transfected construct pENTR-N-FLAG Parry et al., in preparation Lentiviral construct to transfect and express FLAG-tagged autophagy receptors Transfected construct plentiGuide puro Addgene 52963 Lentiviral construction to transfect and expressed guide RNAs Biological sample (M. musculus) Primary bone marrow-derived macrophages, WT C57BL/6J Jackson Laboratory Stock # 000664 Freshly isolated from mice Biological sample (M. musculus) Primary bone marrowdervied macrophages Rosa26-Cas9 knockin mouse Jackson Laboratory (Platt et al., 2014) Stock # 026179 Freshly isolated from mice Antibody anti-beta-actin (Mouse monoclonal) Santa Cruz 47778 WB (1:1000) Antibody Anti-FLAG M2 (Mouse monoclonal) Sigma F3165 WB (1:1000) IF (1:500) Antibody anti-p62 (Mouse monoclonal) Abcam 109012 1 mg/ml Antibody Anti-phospho-Ser172TBK1 (D52C2) Cell signalling 5483 IF (1:400) Antibody Anti-Ubiquitin (FK2) Millipore Sigma 04–263 IF (1:400) Antibody Anti-LC3 (clone 2G6) Nanotools 0260–100/LC3-2G6 IF (1:200) Sequencebased reagent PCR primers IDT PCR primers Please see Supplementary file 2 Commercial assay Luciferase assay system Promega E1500 Commercial kit Mouse IL-6 DuoSet ELISA R and D DY406 Commercial kit Mouse TNF-a DuoSet ELISA R and D DY410 Commercial kit Mouse IL-12/IL-23 p40 allele-specific DuoSet ELISA R and D DY499 Chemical compound, drug Recombinant murine IFN-g Peprotech 315–05 Chemical compound, enzyme Sequencing grade modified trypsin Promega V511 Software, algorithm MaxQuant (Cox and Mann, 2008) MS Database search software Software, algorithm artMS (Jimenez-Morales et al., 2019) MS Statistical analysis software Other DAPI stain Invitrogen D1306 (1 mg/mL) Continued on next page Budzik et al. eLife 2020;9:e51461. .. DOI: https://doi.org/10.7554/eLife.51461 17 of 30 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Strain Mtb: Erdman:: pmv261Zeo_MCherry (Penn et al., 2018) mCherry-expressing Luciferase:Article Title: Dynamic post-translational modification profiling of Mycobacterium tuberculosis-infected primary macrophages Article Snippet: Key resources table Reagent type (species) or resource Designation Source or reference Identifiers Additional information Cell line (M. musculus) RAW 264.7 ATCC TIB-71 Transfected construct pENTR-N-FLAG Parry et al., in preparation Lentiviral construct to transfect and express FLAG-tagged autophagy receptors Transfected construct plentiGuide puro Addgene 52963 Lentiviral construction to transfect and expressed guide RNAs Biological sample (M. musculus) Primary bone marrow-derived macrophages, WT C57BL/6J Jackson Laboratory Stock # 000664 Freshly isolated from mice Biological sample (M. musculus) Primary bone marrowdervied macrophages Rosa26-Cas9 knockin mouse Jackson Laboratory (Platt et al., 2014) Stock # 026179 Freshly isolated from mice Antibody anti-beta-actin (Mouse monoclonal) Santa Cruz 47778 WB (1:1000) Antibody Anti-FLAG M2 (Mouse monoclonal) Sigma F3165 WB (1:1000) IF (1:500) Antibody anti-p62 (Mouse monoclonal) Abcam 109012 1 mg/ml Antibody Anti-phospho-Ser172TBK1 (D52C2) Cell signalling 5483 IF (1:400) Antibody Anti-Ubiquitin (FK2) Millipore Sigma 04–263 IF (1:400) Antibody Anti-LC3 (clone 2G6) Nanotools 0260–100/LC3-2G6 IF (1:200) Sequencebased reagent PCR primers IDT PCR primers Please see Supplementary file 2 Commercial assay Luciferase assay system Promega E1500 Commercial kit Mouse IL-6 DuoSet ELISA R and D DY406 Commercial kit Mouse TNF-a DuoSet ELISA R and D DY410 Commercial kit Mouse IL-12/IL-23 p40 allele-specific DuoSet ELISA R and D DY499 Chemical compound, drug Recombinant murine IFN-g Peprotech 315–05 Chemical compound, enzyme Sequencing grade modified trypsin Promega V511 Software, algorithm MaxQuant (Cox and Mann, 2008) MS Database search software Software, algorithm artMS (Jimenez-Morales et al., 2019) MS Statistical analysis software Other DAPI stain Invitrogen D1306 (1 mg/mL) Continued on next page Budzik et al. eLife 2020;9:e51461. .. DOI: https://doi.org/10.7554/eLife.51461 17 of 30 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Strain Mtb: Erdman:: pmv261Zeo_MCherry (Penn et al., 2018) mCherry-expressing |